Aging is a decline in homeostatic capacity that results in an increased susceptibility to disease and death. Interestingly, there are genetic elements that regulate lifespan in yeast which have been found to have similar effects on mammalian longevity.  Our research into yeast aging aims to unravel the cellular transformations that occur with aging and identify pathways that drive the aging process. This research can help lead to pinpointing potential therapeutic targets for combating age-associated diseases.  Additionally, we are pioneering yeast-based systems that will allow for the discovery of novel medications that can impede known biological pathways implicated in aging, in order to increase healthspan.

Our lab works with medical professionals that identify new suspected human disease-associated mutations.  When a mutation is conserved across species, our lab employs CRISPR genome engineering technology to introduce the suspected disease-associated mutation into the equivalent yeast gene. This allows us to conduct comprehensive genetic screenings and focused phenotypic analysis to deepen our understanding of these under-studied diseases. Presently, we are actively researching mTOR signalling,  the V-ATPase proton pump complex, and the rare disease, porphyria.

We have developed a biochemistry course-based undergraduate research experience (CURE) using CRISPR.  The series of laboratory experiments are designed to equitably provide students with authentic scientific research experiences by allowing students to formulate their own novel hypotheses and to ask questions with unknown answers.  We also develop stand-alone laboratory exercises (e.g., genetic engineering using CRISPR to generate  red yeast) and help other instutitions deploy these exercises with their biology students.  Email (cure at wasko.org) if you are interested in detailed protocols, reagents, etc.